Part:BBa_K2018015:Design
Lacticin Q-Lacticin Z producing
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This hybrid bacteriocin were designed by taking the individual bacteriocin genes, putting them together with a Gly-Gly-Gly hinge in between. The genes were found in the NCBI gene database. The reason for the Glycin hinge in between the two bacteriocins is that it should function as a flexible domain, giving each bacteriocin the possibility to maintain their function.
Why? Our thought behind this hybrid were that maybe two bacteriocins would have a synergistic effect and work together better and more specific than they would on their own.
Literature shows that MIC of Lacticin Q (as a single bacteriocin protein) is 1,800 nM against Staphylococcus aureus:ATCC 12600((Fujita et al. 2007) , in our MIC tests we showed that the hybrid bacteriocin inhibited growth of S. aureus:CC398, USA300 & hVISA at a concentration as low as 642 nM. It even showed antimicrobial effect against Pseudomonas aeruginosa:PAO1 with a MIC value of 1283 nM, an effect that (to our knowledge) neither of the two single bacteriocins has showed effect against.
Source
NCBI genebank database
lacticin Z: Accession: AB298287.1
lacticin Q:Accession: AB235201.1